Yoon, A. (2017). qPCR amplification of heat shock protein 70 in hybrid and pure populations of Tigriopus californicus. Honors Thesis, Louisiana State University, Baton Rouge, Louisiana, USA. 12 pp.

507195

Yoon, A.

2017

12 pp.

Publication

https://repository.lsu.edu/honors_etd/1604

Available for editors   [request]

Global temperatures are continuously rising and becoming a larger threat to the future of numerous species and habitats. Temperature is a critical factor in protein stability and the rate of physiological reactions, being detrimental to an organism if out of its thermal range. The intertidal copepod Tigriopus californicus exhibits local thermal adaptation among populations. Heat shock protein 70 is thought to contribute to the heat tolerance of this organism but it has yet to be determined whether the observed variation in tolerance is due to protein-coding mutations or changes in gene expression. We hybridized two populations of T. californicus and selected for increased heat tolerance. Due to high variation in heat selected samples, qPCR analysis showed no significant difference in fold change in hsp70 expression between the heat selected and control lines, though the raw data showed a trend towards higher fold change in expression in the control lines than the heat selected lines. The selected lines had a positive fold change in hsp70 suggesting that both upregulation and protein coding mutations may be responsible for the heat tolerance of T. californicus.

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