Integrated Marine Information System (IMIS)

Data Policy
Persons | Institutes | Publications | Projects | Datasets
[ report an error in this record ]basket (1): add | show Print this page
one publication added to basket [246865]
Tracking the sterol biosynthesis pathway of the diatom Phaeodactylum tricornutum
Fabris, M.; Matthijs, M.; Carbonelle, S.; Moses, T.; Pollier, J.; Dasseville, R.; Baart, G.J.E.; Vyverman, W.; Goossens, A. (2014). Tracking the sterol biosynthesis pathway of the diatom Phaeodactylum tricornutum. New Phytol. 204(3): 521-535. dx.doi.org/10.1111/nph.12917
In: New Phytologist. Wiley-Blackwell: Oxford. ISSN 0028-646X; e-ISSN 1469-8137
Peer reviewed article  
Available in  Authors 
Keywords
    Bacillariophyceae [WoRMS]; Phaeodactylum tricornutum Bohlin, 1897 [WoRMS]
    Marine/Coastal
Author keywords
    chimeric pathway; diatom; fusion enzymes; isopentenyl diphosphateisomerase; oxidosqualene cyclase; Phaeodactylum tricornutum; squaleneepoxidase; sterol biosynthesis
Authors  Top 
  • Fabris, M.
  • Matthijs, M.
  • Carbonelle, S.
  • Moses, T.
  • Pollier, J.
  • Dasseville, R.
  • Baart, G.J.E.
  • Vyverman, W.
  • Goossens, A.
Abstract
  • Diatoms are unicellular photosynthetic microalgae that play a major role in global primary production and aquatic biogeochemical cycling. Endosymbiotic events and recurrent gene transfers uniquely shaped the genome of diatoms, which contains features from several domains of life. The biosynthesis pathways of sterols, essential compounds in all eukaryotic cells, and many of the enzymes involved are evolutionarily conserved in eukaryotes. Although well characterized in most eukaryotes, the pathway leading to sterol biosynthesis in diatoms has remained hitherto unidentified.
  • Through the DiatomCyc database we reconstructed the mevalonate and sterol biosynthetic pathways of the model diatom Phaeodactylum tricornutum in silico. We experimentally verified the predicted pathways using enzyme inhibitor, gene silencing and heterologous gene expression approaches.
  • Our analysis revealed a peculiar, chimeric organization of the diatom sterol biosynthesis pathway, which possesses features of both plant and fungal pathways. Strikingly, it lacks a conventional squalene epoxidase and utilizes an extended oxidosqualene cyclase and a multifunctional isopentenyl diphosphate isomerase/squalene synthase enzyme.
  • The reconstruction of the P. tricornutum sterol pathway underscores the metabolic plasticity of diatoms and offers important insights for the engineering of diatoms for sustainable production of biofuels and high-value chemicals.
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors 
[Back]

WoRMS

Using WoRMS

Tools

Connect with us

This service is powered by LifeWatch Belgium
Learn more»
Website and databases developed and hosted by Flanders Marine Institute · Page generated on 2024-11-22 08:49:13+01:00 · Privacy and cookie policy